文章摘要
张金山,朱宗恒,王钢胜,等.乳腺癌组织中ING4、HIF-1α和HIF-2α表达及与肿瘤微血管新生的关系[J].安徽医药,2017,21(5):847-850.
乳腺癌组织中ING4、HIF-1α和HIF-2α表达及与肿瘤微血管新生的关系
Expressions of ING4,HIF-1α and HIF-2α in breast cancer tissues andtheir relationship with tumor angiogenesis
投稿时间:2017-01-13  
DOI:
中文关键词: 乳腺癌  生长抑制因子4  缺氧诱导因子-1α  缺氧诱导因子-2α  血管新生
英文关键词: 
基金项目:广东省自然科学基金(2014A030310378)乳腺癌组织中ING4、HIF-1α和HIF-2α表达及与肿瘤微血管新生的关系张金山1,朱宗恒1,王钢胜1,曲少华2 (1.鄂东医疗集团黄石市中心医院肿瘤外科,湖北 黄石 435000;2.中山大学附属第一医院乳腺外科,广东 广州 510000)乳腺癌组织中ING4、HIF-1α和HIF-2α表达及与肿瘤微血管新生的关系 张金山1,朱宗恒1,王钢胜1,曲少华2 Expressions of ING4,HIF-1α and HIF-2α in breast cancer tissues and their relationship with tumor angiogenesis ZHANG Jinshan1,ZHU Zongheng1,WANG Gangsheng1,QU Shaohua2
作者单位
张金山 鄂东医疗集团黄石市中心医院肿瘤外科,湖北 黄石 435000 
朱宗恒 鄂东医疗集团黄石市中心医院肿瘤外科,湖北 黄石 435000 
王钢胜 鄂东医疗集团黄石市中心医院肿瘤外科,湖北 黄石 435000 
曲少华 中山大学附属第一医院乳腺外科,广东 广州 510000 
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中文摘要:
      目的 探讨乳腺癌组织中生长抑制因子4(ING4)、缺氧诱导因子-1α(HIF-1α)和缺氧诱导因子-2α(HIF-2α)表达及与肿瘤微血管新生的关系。方法 选取择期行手术治疗的乳腺癌病人107例,利用实时荧光定量PCR技术检测乳腺癌和癌旁组织中HIF-1α、HIF-2α、ING4和血管内皮生长因子(VEGF)基因表达,免疫组化染色检测乳腺癌和癌旁组织中微血管密度(MVD)。结果 乳腺癌组织中HIF-1α mRNA、HIF-2α mRNA相对表达量均高于癌旁组织,而ING4 mRNA 相对表达量低于癌旁组织,差异有统计学意义(P<0.05);HIF-1α mRNA相对表达量与淋巴结转移有关(P<0.05),HIF-2α mRNA相对表达量与肿瘤大小、淋巴结转移有关(P<0.05),ING4 mRNA相对表达量与临床分期、淋巴结转移有关(P<0.05);乳腺癌组织中VEGF mRNA相对表达量和MVD计数均高于癌旁组织,均差异有统计学意义(P<0.05);Pearson相关分析显示,乳腺癌组织中HIF-1α mRNA、HIF-2α mRNA相对表达量均与VEGF mRNA相对表达量和MVD计数呈正相关(r=0.382、0.417和0.408、0.491,P<0.05),ING4 mRNA相对表达量均与VEGF mRNA相对表达量和MVD计数呈负相关(r=-0.395、-0.502,P<0.05)。结论 乳腺癌组织中HIF-1α、HIF-2α基因表达升高,而ING4基因则被抑制,可能共同参与了乳腺癌组织中血管新生。
英文摘要:
      Objective To investigate the expressions of ING4,HIF-1α and HIF-2α in breast cancer tissues and their relationship with tumor angiogenesis.Methods 107 cases of patients with breast cancer undergoing elective surgery were selected.Expressions of HIF-1α,HIF-2α,ING4 and VEGF genes in breast cancer and adjacent tissues were tested by real-time quantitative PCR.The microvessel density (MVD) in breast cancer and adjacent tissues were detected by using immunohistochemical staining.Results The relative expression levels of HIF-1α mRNA and HIF-2α mRNA in breast cancer tissues were higher than the adjacent tissues,while the relative expression levels of ING4 mRNA was lower than the adjacent tissues.The differences were statistically significant (P<0.05).The relative expression levels of HIF-1α mRNA were correlated with lymph node metastasis (P<0.05).The relative expression levels of HIF-2α mRNA were correlated with tumor size and lymph node metastasis (P<0.05),and the relative expression levels of ING4 mRNA were correlated with clinical stage and lymph node metastasis (P<0.05).The relative expression levels of VEGF mRNA and MVD counts in breast cancer tissues were significantly higher than those in adjacent tissues.The differences were statistically significant (P<0.05).Pearson correlation analysis showed that the relative expression levels of HIF-1α mRNA and HIF-2α mRNA were positively correlated with the relative levels of VEGF mRNA and MVD counts (r=0.382,0.417 and 0.408,0.491,P<0.05),and the expression levels of ING4 mRNA in breast cancer tissues were negatively correlated with the relative expression levels of VEGF mRNA and MVD counts (r=-0.395,-0.502,P<0.05).Conclusion The expressions of HIF-1α and HIF-2α genes in breast cancer tissues were up-regulated and ING4 gene was inhibited,which might be involved in the angiogenesis of breast cancer.
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