文章摘要
顾晓红,顾炳仁,蒋玲俐.高效液相色谱法测定新霉素地塞米松滴眼液中苯扎氯铵的含量[J].安徽医药,2017,21(8):1391-1394.
高效液相色谱法测定新霉素地塞米松滴眼液中苯扎氯铵的含量
The determination of benzalkonium chloride in neomycin dexamethasone eye drops by HPLC
投稿时间:2016-12-06  
DOI:
中文关键词: 苯扎氯铵  新霉素地塞米松滴眼液  高效液相色谱法  含量测定
英文关键词: 
基金项目:
作者单位
顾晓红 苏州市药品检验检测研究中心,江苏 苏州 215104 
顾炳仁 苏州市药品检验检测研究中心,江苏 苏州 215104 
蒋玲俐 南京中医药大学药学院,江苏 南京 210023 
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中文摘要:
      目的 建立了高效液相色谱法(HPLC)测定新霉素地塞米松滴眼液中苯扎氯铵含量的方法。方法 采用C18色谱柱(250 mm × 4.6 mm,5 μm),以0.02 mol·L-1庚烷磺酸钠溶液[含0.1%三乙胺,用磷酸调节pH(3.45±0.1)]-乙腈(20∶80)为流动相,检测波长210 nm,流速1.0 mL·min-1,柱温40 ℃,进样体积20 μL。结果 苯扎氯铵三种同系物在其各自的线性范围内(0.98~126.08、0.85~109.17、0.77~98.25 mg·L-1)内呈良好的线性关系。苯扎氯铵的平均加样回收率为96.53%,RSD为1.75%。结论 该方法准确可靠,操作简单快速,重复性好,灵敏度高,可用于同时测定新霉素地塞米松滴眼液中苯扎氯铵三种同系物的含量,为其安全性考察提供参考。
英文摘要:
      Objective To establish an HPLC method for the determination of benzalkonium chloride in neomycin dexamethasone eye drops. Methods A C18 column (250 mm × 4.6 mm,5 μm) was used as the stationary phase,with 0.02 mol·L-1 sodium heptanesulfonate [containing 0.1% trimethylamine and the pH value was adjusted to(3.45±0.1) by phosphoric acid]-acetonitrile (20∶80) as the mobile phase.The flow rate was 1.0 mL·min-1,the column temperature was 40 ℃ and the sample volume was 20 μL.Detection wavelength was carried out at 210 nm. Results Three homologue benzalkonium chloride in separate its linear range (0.98-126.08,0.85-109.17,0.77-98.25 mg·L-1) had good linear relationship.The average sample recovery rate of benzalkonium chloride was96.53%,with the RSD of 1.75%. Conclusion The method is accurate and reliable,and also with good repeatability and high sensitivity.The operation is simple and fast.It can be suitable for the determination of three benzalkonium chloride homologies in neomycin dexamethasone eye drops,and it provides the reference for the security investigation.
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