文章摘要
曹文婷,冯亮,李莉,等.白芍总苷通过白细胞介素 -6/信号转导及转录激活蛋白 3信号通路对脂多糖诱导的急性肺炎小鼠炎症反应的影响[J].安徽医药,2022,26(1):12-16.
白芍总苷通过白细胞介素 -6/信号转导及转录激活蛋白 3信号通路对脂多糖诱导的急性肺炎小鼠炎症反应的影响
Effects of TGP on the inflammatory responses of LPS-induced acute pneumonia in mice via interleukin-6/signal transducer and activator of transcription 3 signaling pathway
  
DOI:10.3969/j.issn.1009-6469.2022.01.003
中文关键词: 芍药属  白芍总苷  白细胞介素 6/信号转导及转录激活蛋白 3信号通路  脂多糖  肺炎  炎症  急性肺损伤
英文关键词: Paeonia  TGP  Interleukin-6/signal transducer and activator of transcription 3 signaling pathway  LPS  Pneumo-nia  Inflammatory  Acute lung injury
基金项目:
作者单位
曹文婷 青海省交通医院药剂科青海西宁810001 
冯亮 青海省心脑血管病专科医院药剂科青海西宁 810001 
李莉 青海省心脑血管病专科医院药剂科青海西宁 810001 
仁青措毛 青海省心脑血管病专科医院药剂科青海西宁 810001 
李广庭 青海省心脑血管病专科医院药剂科青海西宁 810001 
王海荣 内蒙古自治区国际蒙医医院创新蒙医药工程研究中心内蒙古自治区呼和浩特 010065 
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中文摘要:
      目的探讨白芍总苷( TGP)通过白细胞介素 -6/信号转导及转录激活蛋白 3(IL-6/STAT3)信号通路对脂多糖诱导的急性肺炎小鼠炎症反应的影响。方法本研究起止时间为 2019年 10月至 2020年 2月。选择 SPF级雄性小鼠 50只,采用随机数字表法将小鼠随机分为五组,对照组、急性肺炎模型组、 TGP低剂量组、 TGP中剂量组和 TGP高剂量组。采用苏木精 -伊红( HE)染色观察各组小鼠肺组织病理形态学;采用酶联免疫吸附测定( ELISA)检测各组小鼠肺组织肿瘤坏死因子 -α(TNF-α)、白细胞介素( IL)-1β含量;采用蛋白质印迹( Western blotting)检测各组小鼠肺组织 IL-6、STAT3、磷酸化信号转导及转录激活蛋白 3(p-STAT3)的蛋白表达水平;采用实时荧光定量 PCR(qRT-PCR)检测各组小鼠肺组织 IL-6、STAT3 mRNA表达水平。结果模型组小鼠肺组织结构被破坏,肺泡间隔增厚,大量炎性细胞浸润; TGP药物组病理改变较模型组均存在不同程度改善,白芍总苷高剂量组肺组织仅存在少量炎性细胞浸润。与对照组( 45.12±9.73)ng/L、(21.38±2.13)ng/L相比,模型组及 TGP各剂量组小鼠肺组织 TNF-α(89.30±9.26)ng/L,(84.32±5.08)ng/L,(75.36±4.67)ng/L,(64.06±5.90)ng/L、IL-1β含量( 59.69±3.60)ng/L,(56.87±
英文摘要:
      Objective To investigate the effects of total glucosides of paecony (TGP) on the inflammatory responses of LPS-induced acute pneumonia in mice through interleukin-6/signal transducer and activator of transcription 3 (IL-6/STAT3) signaling pathway.Meth. ods The experimental study started and ended from October 2019 to February 2020. 50 SPF male mice were randomly assigned into 5groups by random number table method: control group, acute pneumonia model group, TGP low dose group, TGP middle dose group andTGP high dose group. Hematoxylin Eosin (HE) staining was used to observe the pathomorphology of lung tissue of mice in each group. Thelevels of tumor necrosis factor α (TNF-α) and Interleukin (IL)-1β in lung tissue of mice in each group were detected by Enzyme Linked Im-munoSorbent Assay (ELISA). Western blotting was used to detect the protein expression level of IL-6, STAT3 and signal transducer and activator of transcription 3 phosphorylation (p-STAT3) in lung tissue of mice in each group. Quantitative Real-time PCR (qRT-PCR) was used to detect the expression of IL-6 and STAT3 mRNA.Results In the model group, the lung tissue structure was destroyed, the alveo-lar septum was thickened, and a large number of inflammatory cells infiltrated; the pathological changes of TGP group were improved indifferent degrees compared with model group, and only a small amount of inflammatory cells infiltrated in the high dose group of TGP.
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