文章摘要
胡绍波,李珊,邹毅,等.血浆细胞周期素依赖性蛋白激酶抑制因子、环腺苷酸反应元件结合蛋白水平与糖尿病视网膜病变关系研究[J].安徽医药,2022,26(1):168-172.
血浆细胞周期素依赖性蛋白激酶抑制因子、环腺苷酸反应元件结合蛋白水平与糖尿病视网膜病变关系研究
Study on relationships between levels of plasma P15INK4b, CREB and diabetic retinopathy
  
DOI:10.3969/j.issn.1009-6469.2022.01.039
中文关键词: 糖尿病视网膜病变  肿瘤坏死因子 α  转化生长因子 β1  血管内皮生长因子类  细胞周期素依赖性蛋白激酶抑制因子  环腺苷酸反应元件结合蛋白  因果律
英文关键词: Diabetic retinopathy  Tumor necrosis factor-alpha  Transforming growth factor beta1  Vascular endothelial growth factors  P15INK4b  Cyclic adenosine monophosphate response element binding protein  Causality
基金项目:
作者单位
胡绍波 仙桃市第一人民医院内分泌科湖北仙桃 433000 
李珊 仙桃市第一人民医院内分泌科湖北仙桃 433000 
邹毅 仙桃市第一人民医院内分泌科湖北仙桃 433000 
孙文忠 仙桃市第一人民医院内分泌科湖北仙桃 433000 
张文君 仙桃市第一人民医院内分泌科湖北仙桃 433000 
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中文摘要:
      目的检测糖尿病视网膜病变( DR)病人血浆中细胞周期素依赖性蛋白激酶抑制因子( P15INK4b)、环腺苷酸反应元件结合蛋白( CREB)水平,探讨 P15INK4b、CREB与 DR发生发展的关系。方法选取 2017年 6月至 2019年 6月仙桃市第一人民医院收治糖尿病(观察组) 198例作为研究对象,其中,非 DR(非 DR组) 95例, DR(DR组) 103例;根据病变阶段将 DR病人分为非增生性糖尿病视网膜病变( NPDR)Ⅰ期组( 24例)、 NPDRⅡ期组( 29例)、 NPDRⅢ期组( 26例)和增生性糖尿病性视网膜病变(PDR)组(24例);同期健康体检者(对照组) 200例作为对照。采集清晨外周血提取血浆、血清,蛋白质免疫印迹(Western blotting)法检测血浆 P15INK4b、CREB水平;酶联免疫吸附(ELISA)法检测血清肿瘤坏死因子 -α(TNF-α)、转化生长因子 β1(TGF-β1)、血管内皮生长因子(VEGF)水平;采用 Pearson法分析 DR病人血浆 P15INK4b、CREB水平与血清 TNF-α、TGF-β1、VEGF水平相关性;采用多因素 logistic回归分析影响 DR发生的因素。结果观察组血浆 P15INK4b、CREB水平较对照组明显升高[( 3.26±1.04)比( 1.71±0.55)(0.84±0.26)比( 0.53±0.16)P<0.05]血清 TNF-α、TGF-β1、VEGF水平较对照组明显升高( P<0.05); DR组血浆
英文摘要:
      Objective To detect the levels of cyclin dependent kinase inhibitors (P15INK4b) and cyclic adenosine monophosphateresponse element binding protein (CREB) in plasma of patients with diabetic retinopathy (DR), and to explore the relationships betweenP15INK4b, CREB and the occurrence, development of DR.Methods A total of 198 patients with diabetes mellitus (observation group)admitted to Xiantao First People's Hospital from June 2017 to June 2019 were selected as the study subjects, including 95 non-DR pa-tients (non-DR group) and 103 DR patients (DR group); DR patients were divided into non-proliferative diabetic retinopathy (NPDR)stage I group (24 cases), NPDR stage Ⅱ group (29 cases), NPDR stage Ⅲ group (26 cases) and proliferative diabetic retinopathy (PDR)group (24 cases); in the same period, 200 healthy people (control group) were taken as control. Peripheral blood was collected in themorning to extract plasma and serum, the levels of P15INK4b and CREB in plasma were detected by Western blotting; the levels of se-rum TNF-α, transforming growth factor-β1 (TGF-β1) and vascular endothelial growth factor (VEGF) were detected by enzyme-linked im-munosorbent assay (ELISA); Pearson method was used to analyze the correlations between levels of plasma P15INK4b, CREB and levelsof serum TNF-α, TGF-β1, VEGF in DR patients; multivariate logistic regression was used to analyze the factors affecting the occurrence of DR.Results The levels of plasma P15INK4b (3.26±1.04) vs. (1.71±0.55), CREB (0.84±0.26) vs. (0.53±0.16), serum TNF-α, TGF-β1 and VEGF in the observation group were significantly higher than those in the control group (P < 0.05); the levels of plasma P15INK4b (4.23±1.39) vs. (2.21±0.72), CREB (1.01±0.33) vs. (0.65±0.21), serum TNF-α, TGF-β1 and VEGF in the DR group were significantly higher than those in the non-DR group (P < 0.05); with the pathological stage upgrading, the levels of plasma P15INK4b, CREB , TNF-α, TGF-β1 and VEGF in DR patients were gradually increased (P < 0.05); the levels of plasma P15INK4b and CREB in DR patients were positively correlated with the levels of serum TNF-α, TGF-β1 and VEGF (P < 0.05); the levels of plasma P15INK4b, CREB, se-rum TNF-α, TGF-β1 and VEGF were all risk factors for DR (P < 0.05).Conclusions The levels of plasma P15INK4b and CREB inpatients with diabetes mellitus are significantly increased, and abnormal expressed with the occurrence and development of DR. Theyare closely related to the levels of serum TNF-α, TGF-β1 and VEGF. They may affect the occurrence and development of DR by inter-acting with TNF-α, TGF-β1 and VEGF. All of them are risk factors for the occurrence of DR, and can be used as markers of the occur-rence and severity of DR.
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