文章摘要
韩欣,丁晓玲,包蕊,等.维生素 B12联合鼠神经生长因子对脑瘫幼鼠智力的改善作用及机制研究[J].安徽医药,2022,26(1):197-201.
维生素 B12联合鼠神经生长因子对脑瘫幼鼠智力的改善作用及机制研究
Effect and mechanism of vitamin B12 combined with mouse nerve growth factor on intelligence improvement of young rats with cerebral palsy
  
DOI:10.3969/j.issn.1009-6469.2022.01.046
中文关键词: 脑性瘫痪  维生素 B12  鼠神经生长因子  智力  机制  大鼠, Wistar 学意,
英文关键词: Cerebral palsy  Vitamin B12  Mouse nerve growth factor  Intelligence  Mechanism  Rats,wistar
基金项目:
作者单位
韩欣 青海省妇幼保健院药剂科青海西宁 810007 
丁晓玲 青海省妇幼保健院药剂科青海西宁 810007 
包蕊 青海省妇幼保健院药剂科青海西宁 810007 
王馨 青海省妇幼保健院药剂科青海西宁 810007 
余涛 青海省妇幼保健院药剂科青海西宁 810007 
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中文摘要:
      目的观察维生素 B12(VB12)联合鼠神经生长因子( MNGF)对脑瘫幼鼠智力的改善作用,并初步探讨其机制。方法研究起止时间为 2018年 1—4月。选取 14 d日龄 Wistar大鼠 72只,按系统随机抽样取 48只建模,余 24只随机分为假手术组和对照组。将建模成功的大鼠随机分为模型组、 VB12组、 MNGF组、 VB12+MNGF组,假手术组仅做切口左侧颈动脉暴露后缝合,对照组无操作。 VB12组神经外膜下注射 VB12注射液 1 mL/100 g,每天 1次; MNGF组给予 MNGF注射液(以无菌生理盐水配置,终浓度为 2 mg/100 mL)1 mL/100 g体质量神经外膜下注射,每天 1次; VB12+MNGF组注射 VB12、MNGF,其余三组仅注射无菌生理盐水,维持 3周。比较各组一般情况变化, Y迷宫实验结果,神经元凋亡指数(AI)脑组织天冬氨酸特异性半胱氨酸蛋白前酶 -3(Pro-Caspase3)、脑组织天冬氨酸特异性半胱氨酸蛋白酶 -3(Caspase3)蛋白表达。结果,对照组大鼠正常饮食、排便且日常活动正常,体质量逐渐增长,假手术组手术后精神萎靡、食欲不佳,后逐渐好转并恢复正常,其余四组均有异常, VB12组、 MNGF组、 VB12+MNGF组干预后情况均好转; Y迷宫实验正确次数从多到少依次为对照组( 19.58±2.07)、 VB12+MNGF组
英文摘要:
      Objective To observe the effect of vitamin B12 (VB12) combined with mouse nerve growth factor (MNGF) on intelli-gence improvement of young rats with cerebral palsy and explore its mechanism.Methods The study started and ended from Januaryto April 2018. A total of 72 Wistar rats aged 14 days old were selected and 48 rats were randomly selected for modeling. The remaining24 rats were randomly divided into sham operation group and control group. The rats were randomly divided into model group, VB12group, mNGF group and VB12 + mNGF group. The control group had no operation. In the VB12 group, 1 mL / 100g of VB12 was inject-ed under the ependyma once a day. In the mNGF group, 1 mL/100g of mNGF (prepared with sterile normal saline, with the final concen-tration of 2 mg/100 mL) was injected under the ependyma once a day. The VB12 + mNGF group was injected with VB12 and mNGF.The other groups were injected with sterile saline, lasting for 3 weeks. The changes of general situation, the Y maze test results, cere-bral cortex apoptosis indexes (AI), the expressions of Pro-Caspase3, Caspase3 proteins in brain tissues were compared.Results The rats in the control group were normal in dieting , urination and daily activities. The rats in the sham operation group had mental depres-sion and poor appetite after operation, and then gradually improved and returned to normal. The other four groups had abnormalities.After intervention, the situation in the VB12 group, the MNGF group and the VB12 + MNGF group improved. Correct number of Y-maze test was (19.58±2.07) in the control group, (17.25±2.18) in the VB12+MNGF group, (13.13±1.76) in the MNGF group, (10.33±
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