| 冯音娇,张丽,韩明静.槲皮素通过调节 FGFR1/TLR4/NLRP3炎症小体途径减轻牙周炎大鼠炎症反应[J].安徽医药,2026,30(6):1086-1091. |
| 槲皮素通过调节 FGFR1/TLR4/NLRP3炎症小体途径减轻牙周炎大鼠炎症反应 |
| Quercetin alleviates the inflammatory response in rats of periodontitis by regulating the FGFR1/TLR4/NLRP3 inflammasome pathway |
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| DOI：10.3969/j.issn.1009-6469.2026.06.006 |
| 中文关键词: 槲皮素 牙周炎 炎症 成纤维细胞生长因子受体1 Toll样受体4 核苷酸结合结构域富含亮氨酸重复序列和含热蛋白结构域受体3 大鼠，Sprague-Dawley |
| 英文关键词: Quercetin Periodontitis Inflammation Fibroblast growth factor receptor 1 Toll-like receptor 4 Nucleotide-binding domain leucine-rich repeat and pyrin domain-containing receptor 3 Rats, Sprague-Dawley |
| 基金项目:四川省医学(青年创新)科研课题项目( S21215) |
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| 中文摘要: |
| 目的探讨槲皮素治疗对牙颈部结扎诱导的牙周炎大鼠牙周组织的影响与具体作用机制。方法该研究于 2024年 1— 6月进行。将 48只 SD大鼠采用随机数字表法均分为对照组、结扎 /脂多糖组、结扎 /脂多糖 +槲皮素( 50 mg/kg)组、结扎 /脂多糖 +槲皮素( 100 mg/kg)组，每组 12只。利用牙颈部结扎法诱导牙周炎大鼠模型，将 10 μL的脂多糖( 1 g/L)注射于下磨牙的下颌骨腭齿龈，每周 2次，连续 7周。利用酶联免疫吸附分析(ELISA)检测血清中肿瘤坏死因子 α(TNF-α)白细胞介素(IL)-1β与 IL-18的浓度。利用 X线分析牙槽骨丢失程度，大鼠麻醉状态下探测牙周袋深度。利用苏木精 -伊红( HE)、染色观察牙周组织的病理变化。利用蛋白质印迹法与免疫荧光染色检测牙周组织中成纤维细胞生长因子受体 1(FGFR1)/Toll样受体 4(TLR4)/核苷酸结合结构域富含亮氨酸重复序列和含热蛋白结构域受体 3(NLRP3)炎症小体通路表达情况。结果与对照组相比，结扎 /脂多糖组血清中 TNF-α、IL-1β与 IL-18水平显著升高( P<0.05)牙周组织中 TLR4、FGFR1、NLRP3、凋亡相关斑点样蛋白质( ASC)与胱天蛋白酶 1(caspase-1)水平显著升高( P<0.05)。与结扎/脂，多糖组相比，结扎 /脂多糖 +槲皮素( 50 mg/kg)组、结扎 /脂多糖 +槲皮素( 100 mg/kg)组大鼠的血清中 TNF-α、IL-1β与 IL-18水平显著降低( P<0.05)，牙周组织中 TLR4(1.64±0.18、1.39±0.17比 2.23±0.22)、 FGFR1(1.52±0.17、1.35±0.15比 1.97±0.21)、 NLRP3(1.57±0.19、1.43±0.15比 2.20±0.28)、 ASC与 caspase-1水平显著降低( P<0.05)且具有剂量依赖性。与对照组相比，结扎 /脂多糖组牙槽骨丢失严重，牙龈组织增生，炎症细胞浸润严重。与结扎/脂多糖组结扎 /脂多糖 +槲皮素组大鼠的牙槽骨丢失与牙周袋深度均减少，牙周组织增生与炎症细胞浸润减轻，且具有剂量依赖性。结论槲皮素可以通过调节 FGFR1/TLR4/NLRP3炎症小体途径抑制炎症反应来减轻牙周炎。 |
| 英文摘要: |
| Objective To investigate the effect and mechanism of quercetin treatment on periodontal tissue in rats with periodontitisinduced by tooth neck ligation.Methods This research was conducted from January to June 2024. Forty-eight SD rats were randomlydivided into four groups by the random number table method: a control group, a ligation/LPS group, a ligation/LPS + quercetin (50 mg/kg) group, and a ligation/LPS + quercetin (100 mg/kg) group, with 12 rats in each group. A rat model of periodontitis was induced bycervical ligation of the teeth. Then, 10 μL of lipopolysaccharide (LPS, 1 g/L) was injected into the palatal gingiva of the mandible at thelower molars twice a week for 7 consecutive weeks. The serum concentrations of tumor necrosis factor-alpha (TNF-α), interleukin-1β (IL-1β), and interleukin-18 (IL-18) were measured by enzyme-linked immunosorbent assay (ELISA). The extent of alveolar bone loss and the depth of periodontal pocket were analyzed by X-ray. Hematoxylin and eosin (HE) staining was used to observe the pathologicalchanges of periodontal tissue. The expression of fibroblast growth factor receptor 1 (FGFR1) / Toll-like receptor 4 (TLR4) / nucleotide-binding domain leucine-rich repeat and pyrin domain-containing receptor 3 (NLRP3) inflammasome pathway in periodontal tissues wasdetected by Western blot and immunofluorescence staining.Results Compared with the control group, the serum levels of TNF-α, IL-1β and IL-18 were significantly increased (P<0.05), and the levels of TLR4 (1.64±0.18, 1.39±0.17 vs. 2.23±0.22), FGFR1 (1.52±0.17, 1.35±0.15 vs. 1.97±0.21), NLRP3 (1.57±0.19, 1.43±0.15 vs. 2.20±0.28), apoptosis-associated speck-like protein (ASC) and caspase-1 in periodontal tissue were significantly increased (P<0.05) in ligation/LPS group. Compared with the ligation/LPS group, the levels of TNF-α, IL-1β and IL-18 in serum of rats were significantly decreased (P<0.05), and the levels of TLR4, FGFR1, NLRP3, ASC and cas. pase-1 in periodontal tissue were significantly decreased (P<0.05) in ligation/LPS + quercetin (50 mg/kg) group and ligation/lipopoly. saccharide + quercetin (100 mg/kg) group in a dose-dependent manner. Compared with the control group, the alveolar bone loss, gingi.val tissue hyperplasia and inflammatory cell infiltration were more serious in the ligation/lipopolysaccharide group. Compared with-liga.tion/lipopolysaccharide group, the alveolar bone loss and periodontal pocket depth of rats in ligation/lipopolysaccharide + quercetingroup were reduced, and the periodontal tissue hyperplasia and inflammatory cell infiltration were alleviated in a dose-dependent man. ner.Conclusion Quercetin can reduce periodontitis by regulating the FGFR1/TLR4/NLRP3 inflammasome pathway to inhibit the in.flammatory response. |
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