| 曾雪莲,赵新,罗晓舟,等.电针与康复训练对脑梗死大鼠运动功能及 Rho/ROCK通路的影响[J].安徽医药,2026,30(6):1097-1102. |
| 电针与康复训练对脑梗死大鼠运动功能及 Rho/ROCK通路的影响 |
| Effects of electroacupuncture and rehabilitation training on motor function and the Rho/ROCK pathway in ischemic stroke rats |
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| DOI：10.3969/j.issn.1009-6469.2026.06.008 |
| 中文关键词: 脑梗死 电针 康复训练 Rho/ROCK通路 运动功能 大鼠， Sprague-Dawley |
| 英文关键词: Ischemic stroke Electroacupuncture Rehabilitation training Rho/ROCK pathway Motor function Rats, Sprague-Dawley |
| 基金项目:四川省医学(青年创新)科研课题项目( S211236) |
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| 中文摘要: |
| 目的探讨电针与康复训练是否可改善脑梗死大鼠运动功能及对 Ras同源蛋白( Rho)/Rho相关蛋白激酶( ROCK)通路的影响。方法 2023年 5月至 2024年 6月，采用随机数字表法从 80只大鼠中选取 65只，采用线栓法建立脑梗死大鼠模型，将其中造模成功的 60只大鼠采用随机数字表法分为脑梗死组、电针组、康复训练组、电针 +康复训练组，每组 15只，剩余未参与造模的 15只大鼠作为假手术组(仅切开颈部皮肤即缝合)。 Zea Longa评分、平衡木行走、转棒转动训练和网屏实验评估大鼠神经和运动功能；酶联免疫吸附分析(ELISA)法检测血清乳酸脱氢酶(LDH)肿瘤坏死因子 α(TNF-α)白细胞介素(IL)-1β、IL-10、神经生长因子( NGF)水平； 2，3，5-氯化三苯基四氮唑( TTC)染色法检测大鼠、脑梗死面积；苏木精 -伊红、(HE)染色观察大鼠缺血侧皮质病理变化；蛋白质印迹法检测大鼠缺血侧皮质组织生长相关蛋白 -43(GAP-43)、脑源性神经营养因子( BDNF)、突触后致密区 95(PSD-95)、 Rho、ROCK1蛋白表达。结果与假手术组相比，脑梗死组大鼠缺血皮质区组织有明显损伤； Zea Longa评分、平衡木行走评分、转棒行走评分和网屏实验评分，血清 LDH、TNF-α和 IL-1β水平，脑梗死面积百分比，缺血侧皮质组织 Rho、ROCK1蛋白表达水平升高；血清 IL-10和 NGF水平，缺血侧皮质组织 GAP-43、BDNF和 PSD-95蛋白表达水平降低( P<0.05)。与脑梗死组相比，电针组、康复训练组和电针 +康复训练组大鼠缺血皮质区组织形态有明显改善； Zea Longa评分、平衡木行走评分、转棒行走评分和网屏实验评分，血清 LDH、TNF-α和 IL-1β水平，脑梗死面积百分比，缺血侧皮质组织 Rho、ROCK1蛋白表达水平降低；血清 IL-10和 NGF水平，缺血侧皮质组织 GAP-43、BDNF和 PSD-95蛋白表达水平升高( P<0.05)。电针 +康复训练对脑梗死大鼠的改善效果［如平衡木行走评分:(1.20±0.16)分比( 1.80±0.27)分、(2.00±0.31)分；缺血侧皮质组织 Rho蛋白: 0.47±0.06比 0.64±0.08、0.65±0.08，ROCK1蛋白: 0.39±0.05比 0.56±0.07、0.58±0.07］优于电针和康复训练单独治疗( P<0.05)。结论电针联合康复训练可能通过抑制 Rho/ROCK信号通路来缓解脑梗死大鼠的炎症反应与脑组织损伤，提升其运动能力。 |
| 英文摘要: |
| Objective To investigate whether electroacupuncture and rehabilitation training can improve motor function in ischemicstroke (IS) rats and the effect on the Ras homolog family member (Rho)/Rho-associated protein kinase (ROCK) pathway. Methods From May 2023 to June 2024, 65 out of 80 rats were selected according to a random number table method to establish the IS rat modelusing the suture method. Among them, 60 rats that were successfully modeled were divided into the IS group, the electroacupuncturegroup, the rehabilitation training group, and the electroacupuncture plus rehabilitation training group according to the random numbertable method, with 15 rats in each group. The remaining 15 rats not involved in modeling served as the sham operation group, in whichonly the neck skin was incised and then sutured. Zea Longa score, balance beam walking, rod rotation training and screen test wereused to evaluate the neurological and motor functions of rats. Serum levels of lactate dehydrogenase (LDH), tumor necrosis factor α(TNF-α), interleukin (IL)-1β, IL-10, and nerve growth factor (NGF) were detected by enzyme-linked immunosorbent assay (ELISA). 2,3, 5-triphenyltetrazolium chloride (TTC) staining was used to detect the infarct size. Hematoxylin and eosin (HE) staining was used to ob.serve pathological changes of the ischemic cortex. Western blotting analysis was performed to detect the protein expression of growth-as. sociated protein-43 (GAP-43), brain-derived neurotrophic factor (BDNF), postsynaptic density protein-95 (PSD-95), Rho, and ROCK1 in the ischemic cortex of rats.Results Compared with the sham group, the ischemic cortical tissue in the IS group was significantlydamaged, and the Zea Longa scores, balance beam walking scores, rod walking scores and screen test scores, serum LDH, TNF-α and IL-1β levels, and percentage of cerebral infarction area were higher, and the expression levels of Rho and ROCK1 proteins in ischemiccortical tissue were increased. The levels of IL-10 and NGF in serum and the protein expressions of GAP-43, BDNF and PSD-95 in the ischemic cortex were decreased (P<0.05). Compared with the IS group, the electroacupuncture group, rehabilitation training group andelectroacupuncture+rehabilitation training group showed significantly improved tissue morphology in the ischemic cortex. The Zea Longascores, balance beam walking scores, rod walking scores, screen test scores, serum LDH, TNF-α and IL-1β levels, percentage of cere.bral infarction area, and the expression levels of Rho and ROCK1 in ischemic cortex were decreased, while the serum levels of IL-10 and NGF and the expression levels of GAP-43, BDNF and PSD-95 in the ischemic cortex were increased (P<0.05). The improvement ef. fect of electroacupuncture plus rehabilitation training on IS rats [balance beam walking score: 1.20±0.16 vs. 1.80±0.27, 2.00±0.31; Rho protein in ischemic cortex: 0.47±0.06 vs. 0.64±0.08, 0.65±0.08; ROCK1 protein: 0.39±0.05 vs. 0.56±0.07, 0.58±0.07] was better than that of electroacupuncture and rehabilitation training alone (P<0.05). Conclusion Electroacupuncture combined with rehabilitationtraining may alleviate the inflammatory response and brain tissue damage and improve motor function in IS rats by inhibiting the Rho/ROCK signaling pathway. |
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