| 杨丹,高阳,热娜 ·买买提.孤独症谱系障碍病儿外周血 Th17细胞比例与血浆 IL-6、 TNF-α、S100B水平的变化及其临床意义[J].安徽医药,2026,30(6):1103-1107. |
| 孤独症谱系障碍病儿外周血 Th17细胞比例与血浆 IL-6、 TNF-α、S100B水平的变化及其临床意义 |
| Changes in peripheral blood Th17 cell proportion and plasma levels of IL-6, TNF-α, and S100B in children with autism spectrum disorder and their clinical significance |
| |
| DOI：10.3969/j.issn.1009-6469.2026.06.009 |
| 中文关键词: 孤独症谱系障碍 白细胞介素 -6 肿瘤坏死因子 α S100钙结合蛋白 B 辅助性 T细胞 17 |
| 英文关键词: Autism spectrum disorder Interleukin-6 Tumor necrosis factor-α S100 calcium-binding protein B Th17 cell |
| 基金项目:新疆维吾尔自治区重点研发计划项目( 2024B03038-2) |
|
| 摘要点击次数: 55 |
| 全文下载次数: 17 |
| 中文摘要: |
| 目的探讨孤独症谱系障碍( ASD)儿童与健康儿童两组外周血 Th17细胞比例，血浆中炎症因子白细胞介素 -6(IL-6)、肿瘤坏死因子 α(TNF-α)、神经损伤标志物 S100钙结合蛋白 B(S100B)水平的变化及其临床意义。方法前瞻性纳入 2024年 6— 12月就诊于新疆医科大学第一附属医院的 3~8岁 ASD病儿 53例作为 ASD组，另纳入同期接诊的健康体检儿童 77例作为健康组。对比组间外周血 Th17细胞比例及血浆 S100B、IL-6、TNF-α水平差异；采用 Pearson相关性分析评估血浆 S100B、IL-6及 TNF-α水平与儿童孤独症评定量表( CARS)评分的相关性；通过曲线下面积( AUC)进一步探讨 S100B、IL-6及 TNF-α对 ASD病儿病情进展及预后的预测效能。结果 ASD组病儿一般资料与健康组相比差异无统计学意义( P>0.05)。与健康组比较， ASD儿童 Th17细胞比例显著升高［( 0.72±0.15)%比( 2.20±0.45)%，P<0.001］； ASD组病儿血浆 S100B、IL-6、TNF-α水平均明显高于健康组［( 249.78±71.80)ng/L比( 31.95±6.30)ng/L、(12.29±2.86)ng/L比( 2.22±2.86)ng/L、(68.06±16.60)ng/L比( 4.41±1.72)ng/L，均 P<0.05］。重度组病儿血浆 S100B、IL-6、TNF-α水平均明显高于轻中度组［( 291.92±73.60)ng/L比( 202.02±24.96)ng/L(14.28±2.02)ng/L比( 10.04±1.79)ng/L、(77.64±12.38)ng/L比( 54.05±11.12)ng/L，均 P<0.05］。 Pearson相关性分析结果表明， ASD病儿血浆中 S100B、IL-6及 TNF-α水平与 CARS评分均呈正相关( r=0.57、0.82、0.68，均 P<0.05)。血浆 S100B、IL-6、TNF-α预测 ASD病情发展的 AUC及其 95%CI分别为 0.81(0.65，0.96)、 0.85(0.70，1.00)、 0.88(0.76，1.00)，特异度分别为 81.20%、87.50%、81.20%，灵敏度分别为 66.70%、80.00%、86.70%；三者联合预测的 AUC及其 95%CI为 0.99(0.97，1.00)，特异度为94.00%，灵敏度为 99.99%；三者联合预测 ASD病情进展的效果、特异度及灵敏度均优于单独预测。结论 ASD病儿外周血 Th17细胞比例较健康儿童升高说明存在免疫失衡。血浆 S100B、IL-6、TNF-α水平在 ASD病儿中表达上调，且与病情严重程度密切相关，有望作为 ASD病情监测及预后评估的潜在外周血标志物。 |
| 英文摘要: |
| Objective To investigate the changes in peripheral blood Th17 cell proportion and plasma levels of the inflammatory cyto. kines interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and the nerve injury marker S100 calcium-binding protein B (S100B)in children with autism spectrum disorder (ASD) compared with healthy controls, and to explore their clinical significance.Methods A prospective study was conducted. The study prospectively enrolled 53 children aged 3-8 years with ASD who visited The First Affili.ated Hospital of Xinjiang Medical University from June to December 2024 as the ASD group, and enrolled 77 healthy children undergo.ing routine physical examinations during the same period as the healthy control group. The differences in peripheral blood Th17 cellproportion and plasma levels of S100B, IL-6, and TNF-α were compared between the two groups. Pearson correlation analysis was used to assess the relationships between plasma S100B, IL-6, TNF-α levels and childhood autism rating scale (CARS) scores. Furthermore,the area under the curve (AUC) was employed to evaluate the predictive value of S100B, IL-6, and TNF-α for disease progression and prognosis in children with ASD.Results There was no statistically significant difference in general data between the ASD group and the healthy control group (P>0.05). Compared with the healthy control group, the proportion of Th17 cells in children with ASD was sig.nificantly increased [(0.72±0.15)% vs. (2.20±0.45)%, P<0.001]. The plasma levels of S100B, IL-6, and TNF-α in the ASD group were significantly higher than those in the healthy control group [(249.78±71.80) ng/L vs. (31.95±6.30) ng/L, (12.29±2.86) ng/L vs. (2.22±2.86) ng/L, (68.06±16.60) ng/L vs. (4.41±1.72) ng/L, all P<0.05]. Furthermore, the plasma levels of S100B, IL-6, and TNF-α in the se. vere ASD subgroup were significantly higher than those in the mild-to-moderate ASD subgroup [(291.92±73.60) ng/L vs. (202.02± 24.96) ng/L, (14.28±2.02) ng/L vs. (10.04±1.79) ng/L, (77.64±12.38) ng/L vs. (54.05±11.12) ng/L, all P<0.05]. Pearson correlation anal. ysis showed that plasma levels of S100B, IL-6, and TNF-α in children with ASD were significantly positively correlated with CARS scores (r=0.57, 0.82, and 0.68, respectively; all P<0.05). The AUCs (95% confidence intervals) for plasma S100B, IL-6, and TNF-α in predicting disease progression of ASD were 0.81 (0.65, 0.96), 0.85 (0.70, 1.00), and 0.88 (0.76, 1.00), respectively; the correspondingspecificities were 81.20%, 87.50%, and 81.20%, and the sensitivities were 66.70%, 80.00%, and 86.70%. The combined prediction us.ing all three markers yielded an AUC of 0.99 (0.97, 1.00), with a specificity of 94.00% and a sensitivity of 99.99%. The combined pre.diction of disease progression in ASD demonstrated superior performance, specificity, and sensitivity compared to any single markeralone (all P<0.05).Conclusions The proportion of Th17 cells in peripheral blood of children with ASD is higher than that of healthychildren, indicating immune imbalance. Plasma levels of S100B, IL-6 and TNF-α are upregulated in children with ASD and are closelyrelated to disease severity, making them potential peripheral blood markers for monitoring disease progression and evaluating prognosisin ASD. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
