文章摘要
李峙玉,翟惠虹.枸杞多糖通过调节肠道菌群缓解溃疡性结肠炎[J].安徽医药,待发表.
枸杞多糖通过调节肠道菌群缓解溃疡性结肠炎
投稿时间:2021-11-29  录用日期:2022-01-11
DOI:
中文关键词: 枸杞多糖  慢性溃疡性结肠炎  肠道菌群  丁酸
英文关键词: 
基金项目:国家自然科学基金(No 8197031585)
作者单位地址
李峙玉 首都医科大学附属北京友谊医院 北京市西城区永安路95号北京友谊医院学生公寓
翟惠虹 首都医科大学附属北京友谊医院 
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中文摘要:
      摘要:目的 探究枸杞多糖(Lycium barbarum polysaccharide,LBP)对慢性溃疡性结肠炎(ulcerative colitis,UC)的保护作用及其对肠道菌群的调节效应。方法 将20只SPF级及20只无菌级(germ-free,GF)健康雄性C57BL / 6小鼠采用随机数表法各分成两组:DSS组(n = 10, SPF级), LBP组(n = 10, SPF级);GF(DSS)组(n = 10, 无菌级), GF(LBP)组(n = 10, 无菌级)。利用三循环间断饮用2%右旋葡聚糖硫酸钠((dextran sulphate sodium,DSS)7日法,建立共54日的慢性溃疡性结肠炎模型。DSS组和GF(DSS)组全程正常饮食,LBP组和GF(LBP)组全程给予1% LBP膳食补充。在第54日,采用应激排便法收集小鼠粪便,16S rRNA高通量测序分析肠道菌群组成, 最后采用气相色谱-质谱联用法检测粪便中短链脂肪酸分子(short-chain fatty acids,SCFAs)含量的变化(本实验自2020年11月1日起至2022年10月1日止)。结果 枸杞多糖显著缓解了结肠炎小鼠的体重、结肠长度、疾病指数评分、髓过氧化物酶活性,但是在无菌级小鼠中枸杞多糖对结肠炎的保护作用消失。16S rRNA测序结果显示,与DSS组相比,LBP组显著增加了益生菌乳杆菌属(0.55×10-2 ± 0.38×10-2 vs 3.83×10-2 ± 1.78×10-2,P = 0.009),毛螺菌属(1.84×10-2 ± 0.42×10-2 vs 3.90×10-2 ±1.36×10-2,P = 0.023),产丁酸球菌属(0.03×10-2 ± 0.04×10-2 vs 0.11×10-2 ±0.01×10-2,P = 0.007)以及unidentified S24-7(9.91×10-2 ± 1.44×10-2 vs 28.90×10-2 ±15.68×10-2,P = 0.004)的丰度。除此之外,枸杞多糖显著增加了结肠炎小鼠粪便中丁酸的含量(3.96 ± 0.78 vs 7.44 ± 1.67,P = 0.022)。但是,枸杞多糖并未增加无菌级结肠炎小鼠的丁酸含量(6.02 ± 0.13 vs 6.05 ± 0.11,P=0.150)。结论 枸杞多糖可能通过调节结肠炎小鼠肠道菌群的组成,促进菌群代谢物丁酸的产生而缓解肠道炎症。
英文摘要:
      Abstract: Objective To investigate the protective effect of Lycium barbarum polysaccharide (LBP) on chronic ulcerative colitis and its regulation effect on intestinal flora. Methods 20 SPF and 20 germ-free healthy male C57BL / 6 mice were respectively divided into two groups by random number table method: DSS group (n = 10, SPF), LBP group (n = 10, SPF); GF (DSS) group (n = 10, germ-free), GF (LBP) group (n = 10, germ-free). The model of chronic ulcerative colitis was established by discontinuously drinking 2% dextran sodium sulfate (DSS) for 7 days with three cycles, totally for 54 days. DSS and GF (DSS) groups were given normal diet, while LBP and GF (LBP) groups were given 1%LBP dietary supplement throughout the whole process. Finally, the feces of mice were collected by stress defecation method, and the composition of intestinal flora was analyzed by 16S rRNA high-throughput sequencing. The content of short-chain fatty acids in feces was detected by gas chromatography-mass spectrometry. Results LBP exerted a significant effect against colitis, as indicated by increased body weight and decreased colon length, DAI scores and myeloperoxidase activity. But the protective effect of LBP on colitis was reversed in germ-free mice. The abundance of some probiotics , such as Lactobacillus 0.55×10-2 ± 0.38×10-2 vs 3.83×10-2 ± 1.78×10-2,P = 0.009), unclassified Lachnospiraceae (1.84×10-2 ± 0.42×10-2 vs 3.90×10-2 ±1.36×10-2,P = 0.023), Butyricicoccus (0.03×10-2 ± 0.04×10-2 vs 0.11×10-2 ±0.01×10-2,P = 0.007), unidentified S24-7(9.91×10-2 ± 1.44×10-2 vs 28.90×10-2 ±15.68×10-2,P = 0.004), increased obviously with LBP treatment. In addition, LBP significantly increased the content of butyric acid in feces of mice with colitis (3.96 ± 0.78 vs 7.44 ± 1.67,P = 0.022). However, LBP did not increase the content of butyric acid in germ-free mice (6.02 ± 0.13 vs 6.05 ± 0.11,P=0.150). Conclusion LBP can alleviate DSS-induced chronic colitis by regulating the composition of intestinal flora and promoting the production of butyric acid
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