王伟,黄永,方增焜,等.亚甲蓝通过脑源性神经营养因子 /原肌球蛋白相关激酶 B通路减轻脑缺血再灌注大鼠的认知功能障碍[J].安徽医药,2024,28(11):2141-2145. |
亚甲蓝通过脑源性神经营养因子 /原肌球蛋白相关激酶 B通路减轻脑缺血再灌注大鼠的认知功能障碍 |
Methylene blue ameliorates cognitive impairment in rats after cerebral ischemia-reperfusion via BDNF/TrkB pathway |
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DOI:10.3969/j.issn.1009-6469.2024.11.005 |
中文关键词: 亚甲蓝 再灌注损伤 认知能力 脑源性神经营养因子 /原肌球蛋白相关激酶 B通路 |
英文关键词: Methylene blue Reperfusion injury Cognitive ability BDNF/TrkB pathway |
基金项目:广西壮族自治区中医药管理局计划课题( GXZYA20220204) |
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中文摘要: |
目的研究亚甲蓝( MB)在缺血再灌注引起的认知功能障碍治疗中的作用及机制。方法 2021年 1月至 2022年 10月,以大脑中动脉栓塞( MCAO)大鼠为实验对象, MCAO术后立即腹腔注射亚甲蓝( 10 mg/kg),运用改良大鼠神经功能缺损评分(mNSS)评估大鼠的神经损伤程度,水迷宫试验评估大鼠的认知能力,尼氏染色观测海马 CA1区神经元结构,脱氧核糖核苷酸末端转移酶介导的缺口末端标记法( TUNEL)检测细胞凋亡情况,蛋白质印迹法检测脑源性神经营养因子( BDNF)/原肌球蛋白相关激酶 B(TrkB)蛋白表达水平。结果与缺血再灌注组相比,缺血再灌注后给予亚甲蓝的大鼠 mNSS评分显著降低[24 h: |
英文摘要: |
Objective To analyze the therapeutic effect of Methylene blue and its mechanism in cognitive impairment after cerebral ischemia-reperfusion. Methods Rats with MCAO surgery were used as experiment subjects from January 2021 to October 2022.mNSS was used to evaluate the neurological damage of rats. Morris water maze test was used to evaluate cognitive ability in rats.Nisslstaining was used to detect neuronal structure in hippocampus CA1 zone in rats. TUNEL was used to detect cell apoptosis. Protein expression of BDNF/TrkB was detected by Western Blotting. Results Compared to the ischemia-reperfusion group, rats administered methylene blue after ischemia-reperfusion exhibited significantly reduced mNSS scores [24 h:(4.63±0.74)points;72 h:(3.5±1.07)points,enhanced cognitive abilities [Morris water maze latency (14.58±2.90)s, crossover platform count 7.25±1.67], decreased infarct volume[(30.54±5.11)mm2],alleviated neuronal structural damage in the hippocampal CA1 region, significantly lower numbers of apoptotic neurons (6.12±2.03), and a notable increase in BDNF/TrkB protein expression levels (BDNF: 0.53±0.01; TrkB: 0.65±0.08). Conclusion Methylene blue ameliorates cognitive impairment in rats after cerebral ischemia-reperfusion via BDNF/TrkB pathway. |
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