黄欢,孙晶,王广利.外周血微 RNA-122-5p和解整合素金属蛋白酶 10在桥本甲状腺炎中的表达及临床意义[J].安徽医药,2024,28(11):2250-2254. |
外周血微 RNA-122-5p和解整合素金属蛋白酶 10在桥本甲状腺炎中的表达及临床意义 |
Expression and clinical significance of peripheral blood miR-122-5p and ADAM10 in patients with Hashimoto's thyroiditis |
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DOI:10.3969/j.issn.1009-6469.2024.11.028 |
中文关键词: 甲状腺炎,自身免疫性 微 RNA-122-5p 解整合素金属蛋白酶 10 甲状腺功能 诊断 |
英文关键词: Thyroiditis,autoimmune MicroRNA-122-5p A disintegrin and metalloproteinase 10 Thyroid function Diagnosis |
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中文摘要: |
目的探究外周血微 RNA-122-5p(miR-122-5p)和解整合素金属蛋白酶 10(ADAM10)在桥本甲状腺炎( HT)病人中的表达情况及临床意义。方法选择 2020年 1月至 2022年 1月在南京医科大学第四附属医院经病理诊断的 HT病人 86例作为 HT组,同期选择经甲状腺腺瘤切除手术的非癌病人 62例作为对照组。收集两组病人的一般资料并检测甲状腺功能。实时荧光定量 PCR(qRT-PCR)检测两组外周血中 miR-122-5p表达水平, ELISA法检测两组外周血中 ADAM10水平。 Pearson法分析 miR-122-5p或 ADAM10水平与甲状腺功能参数的相关性。 logistic回归分析模型评估 HT的影响因素。 ROC曲线用于评估 miR-122-5p和 ADAM10对 HT发生的诊断价值。结果与对照组比较, HT组游离四碘甲状腺原氨酸( FT4)[(8.79±0.85)nmol/L比( 13.15±1.21)nmol/L]、游离三碘甲状腺原氨酸( FT3)[(3.74±0.15)nmol/L比( 4.56±0.24)nmol/L]水平及 ADAM10表达[( 3.21± 1.13)μg/L比( 5.02±1.69)μg/L]降低( P<0.05);促甲状腺激素( TSH)、甲状腺过氧化物酶抗体( TPOAb)、甲状腺球蛋白抗体(TGAb)、 C反应蛋白( CRP)水平及 miR-122-5p表达( 1.57±0.46比 1.02±0.34)均升高( P<0.05)。 HT组和对照组 miR-122-5p与 TGAb、TPOAb、CRP水平呈正相关( P<0.05); ADAM10与 TGAb、TPOAb及 CRP均呈负相关( P<0.05)。 HT组中 miR-122-5p与 ADAM10水平呈负相关(r=?0.46,P<0.05)。MiR-122-5p、ADAM10、TGAb和 TPOAb是 HT发生的影响因素(P<0.05)。MiR-122-5p、 ADAM10及二者联合区分 HT组和对照组的最佳截断值分别为 1.22、4.74 μg/L、0.42,曲线下面积分别为 0.83(0.76,0.89)、 0.85(0.78,0.90)、 0.91(0.85,0.95)。结论 HT病人外周血中 miR-122-5p表达升高, ADAM10水平降低,二者与甲状腺微结构损伤有关,且对 HT的早期诊断具有一定价值。 |
英文摘要: |
Objective To investigate the expression and clinical significance of peripheral blood microRNA-122-5p (miR-122-5p) and a disintegrin and metalloproteinase 10 (ADAM10) in patients with Hashimoto's thyroiditis (HT).Methods From January 2020 toJanuary 2022, 86 HT patients who were pathologically diagnosed in the Fourth Afiliated Hospital of Nanjing Medical University wereregarded as the HT group. During the same period, 62 non-cancer patients who underwent thyroid adenoma resection were regarded asthe control group. The general data of patients in the two groups were collected and thyroid function parameters were detected. Quantitative real-time PCR (qRT-PCR) was applied to analyze the expression level of miR-122-5p in the peripheral blood of the two groups, andELISA was used to detect the level of ADAM10 in peripheral blood of the two groups. Pearson method was applied to analyze the correlation of miR-122-5p or ADAM10 levels with thyroid function parameters. logistic regression analysis model was applied to evaluate theinfluencing factors of HT. ROC curve was used to evaluate the discriminative value of miR-122-5p and ADAM10 for the HT group and the control group.Results Compared with the control group, the levels of free thyroxin (FT4)[(8.79±0.85)nmol/L vs. (13.15±1.21)nmol/ L], free triiodothyronine (FT3) [(3.74±0.15)nmol/L vs. (4.56±0.24)nmol/L] and the expression of ADAM10[(3.21±1.13)μg/L vs. (5.02± 1.69)μg/L] in the HT group were decreased (P<0.05); the levels of thyroid-stimulating hormone (TSH), thyroid peroxidase antibody (TPOAb), thyroglobulin antibody (TGAb), C-reactive protein (CRP) and miR-122-5p expression(1.57±0.46 vs. 1.02±0.34) were all increased (P<0.05). MiR-122-5p was positively correlated with the levels of TGAb, TPOAb and CRP in HT group and control group (P<0.05); ADAM10 was negatively correlated with TGAb, TPOAb and CRP (P<0.05). There was a negative correlation between miR-1225p and ADAM10 levels in the HT group (r=?0.46, P<0.05). MiR-122-5p, ADAM10, TGAb and TPOAb were the influencing factors of HT (P<0.05). The optimal cutoff values for distinguishing between the HT group and the control group were 1.22, 4.74 μg/L, and 0.42for MiR-122-5p, ADAM10, and their combination. The area under the curve was 0.83(0.76,0.89), 0.85(0.78,0.90), and 0.91(0.85,0.95),respectivelyConclusion The expression of miR-122-5p in peripheral blood of HT patients is increased, and the level of ADAM10 isdecreased, both of which are related to thyroid microstructure damage, and have certain value for the early diagnosis of HT. |
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