| 曾双,黄永.Maresin 1抑制缺血再灌注大鼠脑细胞凋亡及炎症反应[J].安徽医药,待发表. |
| Maresin 1抑制缺血再灌注大鼠脑细胞凋亡及炎症反应 |
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| 投稿时间：2024-09-04 录用日期：2024-10-10 |
| DOI： |
| 中文关键词: Maresin 1 缺血再灌注 GPX4 SLC7A11 |
| 英文关键词: |
| 基金项目:国家自然科学基金（82260961、8206150510），广西重点研发计划 桂科AB18126085 |
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| 中文摘要: |
| 【摘要】目的：研究Maresin 1在缺血再灌注脑组织中的作用及机制。 方法：以大脑中动脉闭塞模型造模缺血再灌注大鼠作为实验对象，通过运用改良大鼠神经功能缺损评分mNSS来评估大鼠的运动功能，利用脱氧核糖核苷酸末端转移酶介导缺口末端标记法（TUNEL法）来检测细胞凋亡的情况，q-PCR检测炎性因子IL-1β、TNF-α和IL-6的表达，Western Blot检测铁死亡通路蛋白GPX4和SLC7A11的表达水平。结果：缺血再灌注后给予Maresin 1治疗的大鼠mNSS评分显著降低；梗死面积显著减少，细胞凋亡数量显著下降，炎性因子IL-1β、TNF-α和IL-6的含量下降，铁死亡通路相关蛋白GPX4和SLC7A11的表达量显著升高。结论：Maresin 1能够减轻缺血再灌注大鼠脑组织损伤，减轻凋亡及炎症反应，并且有可能通过调控铁死亡通路实现的。 |
| 英文摘要: |
| 【Abstract】Objective: To investigate the role and mechanism of Maresin 1 in ischemia-reperfusion brain tissue. Methods: We used a middle cerebral artery occlusion model to induce ischemia-reperfusion in rats. We assessed motor function using the modified Neurological Severity Score (mNSS). We measured apoptosis using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). We assessed the expression of inflammatory factors IL-1β, TNF-α, and IL-6 via q-PCR. Western Blot analysis evaluated the expression levels of ferroptosis-related proteins GPX4 and SLC7A11. Results: In rats treated with Maresin 1 after ischemia-reperfusion, mNSS scores significantly decreased. The infarct area significantly reduced, the number of apoptotic cells markedly decreased, and the levels of inflammatory factors IL-1β, TNF-α, and IL-6 diminished. Meanwhile, the expression of ferroptosis-related proteins GPX4 and SLC7A11 significantly increased. Conclusion: Maresin 1 mitigates brain tissue damage in ischemia-reperfusion in rats. It reduces apoptosis and inflammatory responses, potentially through the regulation of the ferroptosis pathway. |
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