刘颖业,郭巍巍.外周血淋巴细胞亚群测定在儿童传染性单核细胞增多症中的变化及临床意义[J].安徽医药,待发表. |
外周血淋巴细胞亚群测定在儿童传染性单核细胞增多症中的变化及临床意义 |
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投稿时间:2024-09-09 录用日期:2024-10-28 |
DOI: |
中文关键词: 儿童 传染性单核细胞增多症 淋巴细胞亚群 肝功能 |
英文关键词: |
基金项目:河北省2020年度医学科学研究课题计划(No 20200676) |
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中文摘要: |
目的 探讨淋巴细胞亚群在儿童传染性单核细胞增多症(IM)中的特点,为早期诊断提供理论参考依据。方法 选取2022年1月至2023年12月我院收治的IM患儿62例,依据是否出现肝功能异常,分为肝功能正常组(n=31)和肝功能异常组(n=31)。同时选取健康儿童31例作为空白对照组。采用流式细胞术检测患儿外周血淋巴细胞亚群的百分比和表达数量,分析淋巴细胞亚群指标(CD3+、CD3+CD4+、CD3+CD8+、CD3+CD4+/CD3+CD8+、CD19+、CD16+56+)的水平变化及意义。结果 IM组患儿CD3+细胞的百分比、CD3+CD8+细胞的百分比和显著高于健康对照组,而CD3+CD4+细胞的百分比、CD19+细胞百分比、CD16+56+细胞百分比和CD3+CD4+/CD3+CD8+比值显著低于健康对照组,以上指标的变化,差异具有统计学意义(P<0.05)。肝功能异常组患儿CD3+细胞的百分比86.72(81.36,89.98),显著高于肝功能正常组的84.29(78.58,87.04),而CD19+的百分比为3.62(2.89,6.62),显著低于肝功能正常组的5.33(4.45,9.17)。肝功能异常组患儿CD3+细胞的绝对值和CD3+CD8+细胞的绝对值分别为6014(5077,11164)个/μL和4614(3220,8103)个/μL,显著高于肝功能正常组的4798(3166,7140)个/μL和2962(2401,4918)个/μL。差异具有统计学意义(P<0.05)。结论 外周血淋巴细胞亚群测定在儿童IM合并肝功能异常的病情评估中发挥着重要作用,监测淋巴细胞亚群指标的百分比和计数的变化,能为临床早期诊断IM提供有力的科学依据,有助于减少并发症的发生风险,进一步改善患儿的预后情况。 |
英文摘要: |
Objective To explore the characteristics of lymphocyte subsets in infectious mononucleosis (IM) in children and provide a theoretical reference basis for early diagnosis. Methods 62 children with IM admitted to our hospital from January 2022 to December 2023 were selected. According to whether there were abnormal liver function, they were divided into normal liver function groups (n=31) and liver dysfunction groups (n=31). At the same time, 31 healthy children were selected as a control group. Flow cytometry was used to detect the percentage and expression of peripheral blood lymphocyte subsets in children, and the horizontal changes and significance of lymphocyte subset indicators (CD3+, CD3+CD4+, CD3+CD8+, CD3+CD4+/CD3+CD8+, CD19+, CD16+56+) were analyzed. Results The percentage of CD3+ cells and the percentage of CD3+CD8+ cells in children in the IM group were significantly higher than that those in the healthy control group, while the percentage of CD3+CD4+ cells, CD19+ cells, CD16+56+ cells and the CD3+CD4+/CD8+ ratio were significantly lower (P<0.05). In the liver dysfunction group, the percentage of CD3+ cells was 86.72 (81.36,89.98), which was significantly higher than 84.29 (78.58,87.04) in the normal liver function group, while the percentage of CD19+ was 3.62 (2.89, 6.62), which was significantly lower than that of 5.33 (4.45, 9.17) in the normal liver function group. The absolute values of CD3+ cells and CD3+CD8+ cells in the liver dysfunction group were 6014 (5077,11164)/μL and 4614 (3220, 8103)/μL, respectively, significantly higher than the 4,798 (3166,7140)/μL and 2962 (2401, 4918)/μL in the normal liver function group. Differences are statistically significant (P<0.05). Conclusion Peripheral blood lymphocyte subset determination plays an important role in evaluating IM in children with liver function abnormalities. Monitoring the percentage and count of lymphocyte subset indicators can provide a basis for early clinical diagnosis, reduce the occurrence of complications, and improve prognosis. |
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